Diagnostics for sexually transmitted infections (STIs) with easy non-invasive sample collection are important to increase testing and hence to reduce the spread of this infection. From this point of view, molecular technologies and rapid point-of-care tests are innovations which have gradually shifted the paradigm in the diagnosis of STIs from biological to molecular amplification and from laboratory to near-patient testing. These powerful diagnostic tools have improved and will continue to have a significant impact on our ability to design strategies and programs for the control and prevention of STIs.