Tuberculosis of the penis is rare. The clinical features of penile tuberculosis are usually manifested as ulceration or scars. However, the authors encountered a case of penile tuberculosis that presented as a mass. A painless nodule at the base of the penis was noted in a 63-year-old male patient. Surgical excision was recommended, and pathologic finding revealed granulomatous inflammation in the mass. Acid fast bacilli stain and culture were negative, but a positive result was found in urine polymerase chain reaction for detection of Mycobacterium tuberculosis. He was diagnosed with tuberculosis of the penis and underwent anti-tuberculosis chemotherapy.